RESUMO
BACKGROUND: Human papilloma virus (HPV) is one of the most frequent sexually transmitted infections, especially among young people. AIMS: To describe the prevalence of genital HPV infections, to identify clinical-epidemiological factors associated with them and to determine the frequency of viral strains. METHODS: Endocervical samples were studied of 505 women between 15 and 49 years old, who attended the laboratory of the Institute of Social Security, residents of Posadas, Misiones, for the study of vaginal exudate, between January 2012 and June 2013. A 450-base pair fragment within the HPV L1 region was amplified. Genotype detection was performed through the study of the restriction fragment length polymorphism (RFLP). RESULTS: The prevalence of total HPV carriage was 30.7%, and of these 71.6% corresponded to high risk (mainly HPV 16 [35.1%], 58 [10.8%] and 31 [8.1%]). HPV detection was higher in women aged 15 to 24 years (OR: 1.48, 95% CI: 1.01-2.18) and with more sexual partners (OR: 1.81, 95% CI: 1, 02-3.22). There was no association with pregnancy, stability of the couple, contraceptive methods, age at onset of sexual intercourse, or smoking. CONCLUSIONS: The identification and typing of HPV in this study provides information regarding the high prevalence of HPV and the substantial proportion of cases with oncogenic genotypes among sexually active women in this region of Argentina.
Assuntos
Seguro Saúde , Adolescente , Adulto , Argentina , DNA Viral , Feminino , Humanos , Pessoa de Meia-Idade , Papillomaviridae , Infecções por Papillomavirus , Gravidez , Prevalência , Fatores de Risco , Adulto JovemRESUMO
Resumen Introducción: El virus papiloma humano (VPH) causa infecciones transmitidas sexualmente. Objetivos: Conocer la prevalencia de infecciones genitales por VPH, identificar factores clínico-epidemiológicos asociados a dicha prevalencia y determinar la frecuencia de los tipos virales. Material y Métodos: Se estudiaron muestras endo-cervicales de 505 mujeres entre 15 y 49 años, que concurrieron para estudio de exudado vaginal al laboratorio del Instituto de Previsión Social, residentes de Posadas, Misiones, entre enero de 2012 y junio de 2013. Se amplificó una sección de 450 pares de bases del genoma viral perteneciente al fragmento L1 del VPH. La detección del genotipo se realizó mediante el estudio del polimorfismo de la longitud de los fragmentos de restricción (RFLP). Resultados: La prevalencia de la portación de VPH total fue 30,7%, y de éstos 71,6% correspondió a los de alto riesgo (principalmente VPH 16 [35,1%], 58 [10,8%] y 31 [8,1%]). La detección del VPH fue mayor en mujeres de 15 a 24 años (OR: 1,48; IC 95% :1,01-2,18) y con más parejas sexuales (OR:1,81; IC 95%: 1,02-3,22). No hubo asociación con el embarazo, la estabilidad de la pareja, métodos anticonceptivos, edad de inicio de las relaciones sexuales ni tabaquismo. Conclusiones: Los resultados de identificación y tipificación de VPH en este estudio aportan información sobre una prevalencia elevada de VPH en mujeres sexualmente activas, así como porcentajes elevados de genotipos oncogénicos en esta región.
Abstract Background: Human papilloma virus (HPV) is one of the most frequent sexually transmitted infections, especially among young people. Aims: To describe the prevalence of genital HPV infections, to identify clinical-epidemiological factors associated with them and to determine the frequency of viral strains. Methods: Endocervical samples were studied of 505 women between 15 and 49 years old, who attended the laboratory of the Institute of Social Security, residents of Posadas, Misiones, for the study of vaginal exudate, between January 2012 and June 2013. A 450-base pair fragment within the HPV L1 region was amplified. Genotype detection was performed through the study of the restriction fragment length polymorphism (RFLP). Results: The prevalence of total HPV carriage was 30.7%, and of these 71.6% corresponded to high risk (mainly HPV 16 [35.1%], 58 [10.8%] and 31 [8.1%]). HPV detection was higher in women aged 15 to 24 years (OR: 1.48, 95% CI: 1.01-2.18) and with more sexual partners (OR: 1.81, 95% CI: 1, 02-3.22). There was no association with pregnancy, stability of the couple, contraceptive methods, age at onset of sexual intercourse, or smoking. Conclusions: The identification and typing of HPV in this study provides information regarding the high prevalence of HPV and the substantial proportion of cases with oncogenic genotypes among sexually active women in this region of Argentina.
Assuntos
Humanos , Feminino , Gravidez , Adolescente , Adulto , Pessoa de Meia-Idade , Adulto Jovem , Seguro Saúde , Papillomaviridae , Argentina , DNA Viral , Prevalência , Fatores de Risco , Infecções por PapillomavirusRESUMO
Introducción. Chlamydia trachomatis es una de las bacterias más prevalentes en infecciones sexualmente transmisibles. En mujeres, la infección por C. trachomatis puede causar cervicitis y uretritis, aunque suele cursar en forma asintomática. El objetivo de este trabajo fue establecer la prevalencia de C. trachomatis y detectar los genotipos circulantes en mujeres que concurren al laboratorio del Instituto de Previsión Social. Material y métodos. Se analizaron 505 muestras endocervicales de mujeres sintomáticas y asintomáticas. Se determinó la presencia de C. trachomatis por PCR, mediante la amplificación de un fragmento del plásmido críptico. Las muestras positivas fueron genotipificadas mediante la amplificación parcial del gen ompA y se analizaron filogenéticamente. Resultados. Se detectaron 43 muestras positivas a la infección por C. trachomatis, obteniéndose una prevalencia de 8,5% (IC 95%: 6,4-11,3%). La prevalencia de C. trachomatis fue superior en las mujeres con sintomatología vaginal [11,3% (30/265) vs. 5,4% (13/240)] (p=0,018), así como en las mujeres menores de 26 años [11,5% (28/244) vs. 6,1% (15/246)] (p=0,021). A partir del análisis filogenético, se observó que el 62% de las muestras positivas para el gen ompA pertenecieron al genotipo E, 15% al genotipo J, 15% al genotipo D y 8% al F. Conclusiones. Este trabajo es el primer aporte sobre la epidemiología molecular de C. trachomatis en la provincia de Misiones, Argentina, que muestra la tasa de prevalencia de esta bacteria y ofrece información de genotipos circulantes (AU)
Background. Chlamydia trachomatis is the most prevalent bacteria causing sexually transmitted infections. In women, this infection can cause cervicitis and urethritis, although its usually asymptomatic. The aim of this study was to investigate the prevalence of C. trachomatis in women attending the lab Instituto de Previsión Social and detect the genotypes. Material and methods. Endocervical samples from 505 symptomatic and asymptomatic women were assayed. It was determined the presence of C. trachomatis by PCR through amplification of a fragment of the cryptic plasmid. Positive samples were genotyped by the partial amplification of the ompA gene and analyzed phylogenetically. Results. Forty-three positive samples were detected to infection with C. trachomatis, obtaining a prevalence of 8.5% (IC 95%: 6.4-11.3%). The prevalence of C. trachomatis was higher in women with vaginal symptoms [11.3% (30/265) vs. 5.4% (13/240)] (p = 0.018), as well as in women under 26 year-old [11.5% (28/244) vs. 6.2% (15/246)] (p = 0.021). Based on phylogenetic analysis, it was observed that 62% of the samples were genotype E, 15% genotype J, 15% genotype D, and 8% genotype F. Conclusions. This work is the first contribution on the molecular epidemiology of C. trachomatis in the Misiones province, Argentina, which shows the rate of prevalence of this bacterium and offers information on circulating genotypes (AU)
Assuntos
Humanos , Feminino , Adolescente , Adulto Jovem , Adulto , Pessoa de Meia-Idade , Infecções por Chlamydia/epidemiologia , Chlamydia trachomatis/isolamento & purificação , Técnicas de Genotipagem , Argentina/epidemiologia , Chlamydia trachomatis/genética , Filogenia , Esfregaço Vaginal , Reação em Cadeia da Polimerase , Doenças Bacterianas Sexualmente Transmissíveis/epidemiologiaRESUMO
Estudio descriptivo exploratorio de la opinión del personal de enfermería y usuarios internados en el sevicio de Clínica Médica del Hospital Central del IPSsobre la calidad de atención brindada. Presenta conceptos relacionados a la calidad en la atención brindada, de los servicios y el papel de la enfermera dentro de su perfil profesional
